Journal: Scientific Reports

Article Title: Extracellular vesicles from Paracoccidioides pathogenic species transport polysaccharide and expose ligands for DC-SIGN receptors

doi: 10.1038/srep14213

Figure Lengend Snippet: Data were normalized and the scale is indicated below the figures. ( a ) Unsupervised hierarchical clustering was performed for individual technical replicates. ( b ) Competitive inhibition of EV interactions with lectin microarray with mannose (Man) and N -acetylglucosamine (GlcNAc). ( c ) Lectin microarray of PNGase F-treated EVs. Significance (* = p ≤ 0.05) was determined by the ANOVA test.

Article Snippet: Probes (neoglyconjugates, glycoproteins and lectins) were printed on Nexterion® Slide H (Schott, Mainz, Germany) using a SciFlexArrayer S3 piezoelectric microarray printer (Scienion, Berlin, Germany) under constant 62% (+/−2%) humidity at 20 °C essentially as previously described .

Techniques: Inhibition, Microarray

Journal: Molecular Pharmaceutics

Article Title: High-Throughput Microarray Approaches for Predicting the Stability of Drug–Polymer Solid Dispersions

doi: 10.1021/acs.molpharmaceut.4c00955

Figure Lengend Snippet: Comparing the Printed Volume and Deposited Mass of Two Techniques, the Drop- Cast Method and 2D Inkjet Printing

Article Snippet: A miniaturized, high-throughput technique was used to print microarrays of the drug–polymer mixtures using a piezoelectric inkjet printer Sciflexarrayer S5, Scienion (inkjet printer software sciflexarrayer (Scienion A, version 2.09.002)), and 70 μm orifice nozzle type 2 coating (Scienion, Germany).

Techniques: Formulation, Polymer

Journal: ACS Omega

Article Title: Differential Glycosylation Expression in Injured Rat Spinal Cord Treated with Immunosuppressive Drug Cyclosporin-A

doi: 10.1021/acsomega.8b02524

Figure Lengend Snippet: Intensity of primary astrocyte and Neu7 cell protein extracts binding to lectins on microarray. Bar chart representing the differences in binding of fluorescently labeled protein extracts to printed lectins on a microarray surface where Ast_Lys is the total lysate from primary astrocytes, Ast_Cyt is the cytosolic (hydrophilic) protein-enriched extract from primary astrocytes, Ast_Mem is the membrane (hydrophobic) protein-enriched extract from primary astrocytes, Neu7_Lys is the total lysate from Neu7 cells, Neu7_Cyt is the cytosolic (hydrophilic) protein-enriched extract from Neu7 cells, and Neu7_Mem is the membrane (hydrophobic) protein-enriched extract from Neu7 cells. Graph represents the mean of three replicate experiments (except for duplicate experiments for Neu7_Mem binding to MAL-II), with each experiment the median of six individual replicates. Error bars represent ±1 standard deviation of the mean of the three experiments.

Article Snippet: The lectins were printed at approximately 1 nL per feature on Nexterion Slide H microarray slides using a sciFLEXARRAYER S3 piezoelectric printer (Scienion AG, Berlin, Germany) as previously described.

Techniques: Binding Assay, Microarray, Labeling, Standard Deviation

Journal: Molecular Pharmaceutics

Article Title: High-Throughput Microarray Approaches for Predicting the Stability of Drug–Polymer Solid Dispersions

doi: 10.1021/acs.molpharmaceut.4c00955

Figure Lengend Snippet: Schematic representation of steps followed in manufacturing and analyzing microarrays, including drug and polymer solutions preparation, pipetting into well-plate, withdrawing drug solution through the printer nozzle and then dispensing at the required amount in the desired prelocated place on the substrate followed by repeating the same process for the polymer solution to print it onto the printed drug solution droplets.

Article Snippet: A miniaturized, high-throughput technique was used to print microarrays of the drug–polymer mixtures using a piezoelectric inkjet printer Sciflexarrayer S5, Scienion (inkjet printer software sciflexarrayer (Scienion A, version 2.09.002)), and 70 μm orifice nozzle type 2 coating (Scienion, Germany).

Techniques: Polymer

Journal: Molecular Pharmaceutics

Article Title: High-Throughput Microarray Approaches for Predicting the Stability of Drug–Polymer Solid Dispersions

doi: 10.1021/acs.molpharmaceut.4c00955

Figure Lengend Snippet: Examples of different APIs/PVPVA printed dispersions. All images are reported without a cross-polarized filter (top part of each section) and with a cross-polarized filter (bottom part). The images were arranged in ascending order according to the displayed drug–polymer ratio. The top section shows the API/polymer loading ratios with no birefringence (no crystallization monitored). In contrast, the middle section shows the API/polymer loading ratios that show the start of birefringence detection (start of crystallization), and the bottom section shows the API/polymer loading ratios that show apparent birefringence detection (crystallization). Three different ratios for API/polymer loading were displayed to compare the assessment of crystallization by POM. All images are reported after storage of the microarrays in accelerated conditions for 6 months (75% relative humidity and 40 °C in stability oven). Final mass: 1000–1100 ng.

Article Snippet: A miniaturized, high-throughput technique was used to print microarrays of the drug–polymer mixtures using a piezoelectric inkjet printer Sciflexarrayer S5, Scienion (inkjet printer software sciflexarrayer (Scienion A, version 2.09.002)), and 70 μm orifice nozzle type 2 coating (Scienion, Germany).

Techniques: Polymer, Crystallization Assay